Objective: We conveyed 10 year interval survey, named Korean Internet Sexuality Survey (KISS) 2014 to investigate the prevalence and the risk factors of Female Sexual Dysfunction (FSD) in Korea. Design and Method: The Internet-based survey was performed in 20-59 year old women via e-mail and they were asked to answer the questionnaire only if they were sexually active, having sex more than once per month during the last 6 months, following the same method of our previous study in 2004. Results: In total, 516 responses were analyzed. The mean age of the subjects was 38.1±10.3. We defined FSD as FSFI total score under 25.0 and the prevalence of FSD was 46.7%. The prevalence of FSD in each age group increased with age: 23.7% in 20s, 45.4% in 30s, 51.4% in 40s and 72% in 50s. The prevalence of FSD in 20s decreased significantly from 44.3% in 2004 to 23.7% in 2014 (p-value=0.000). All six domains of FSD increased with age. Especially, lubrication and pain domain dysfunctions were highly prevalent in 50s. The risk factors for FSD were analyzed as constipation, Beck Depression Inventory (BDI) and Overactive Bladder Symptom Score (OABSS). Women with older age at first intercourse, increasing number of sexual partners and use of contraceptive pill had lower risk to have FSD. Conclusions: FSD was still highly prevalent in Korea and it increased with age. Compared to the previous study, prevalence of FSD in 20s significantly decreased. Further periodic study is necessary to widen the scope of understanding FSD.