Objective: Sexual dysfunction is an important public health concern. Sexual dysfunctions, characterized by disturbances in sexual desire, arousal, erection, orgasm or sexual satisfaction have been reported in different research around the world. Prevalence range of disturbances is from 20% to 30% and 40% to 45% for men and women respectively. The aim of this study was to explore prevalence of sexual dysfunction in a Colombian male and female sample. Design and Method: Sample was composed by a non-representative national sample of 1366 women and 1410 men, aged from 18 to 73. To assess sexual dysfunction the Colombian version of the Massachusetts General Hospital- Sexual Functioning Questionnaire (MGH-SFQ) was used. Results: Results indicate on one hand that sexual dysfunction is more prevalent in women (42.5%) than men (32.1 %). On the other hand, in women, problems related to desire (28.5%) were more common, while in men those related to desire and sexual satisfaction, 21.4% and 21% respectively, were more prevalent. In women, problems related to desire (28.5%) were more common, while in men those related to desire and sexual satisfaction 21.4% and 21%, respectively were more prevalent. Furthermore, no evidence was found about the relationship between the sociodemographic characteristic (socioeconomic level, years of education and religion) and the sexual dysfunction; with exception of age. These results are similar to those observed in other cultures. Conclusions: This study shows the importance of epidemiologic research in male and female sexual dysfunction for the identification of risk factors.