Objective: There is a social and public health concern about the increasing incidence of sexual violence recently in Korea. Although it is essential to train practitioners to effectively help victim’s treatment and recovery, the training program to strengthen the professionality has not been well-developed yet. This study aims to examine practitioners’ professional training needs and expectations for improving their skills. Design and Method: Among the entire 205 centers for the victims of sexual assault (public 34, private 171) in 17 regional areas of Korea, 19 practitioners from 8 centers (public 4, private 4) from 5 regions participated in 5 sessions of focus group interview (FGI). With the results of FGI, we developed the questionnaire for the needs assessment of practitioners and surveyed. 484 practitioners in 90 institutions agreed to participate in this needs assessment. Results: Practitioners’ professional training needs identified with the questionnaire were as follows. 1) The strengthening the professionality of each service provider (medical doctor, nurse, social worker, psychologist, legal associate and etc) is highest percentage (24.6%, N=239). 2) Improving mental health and prevention of burnout are second interest (15.2%, N=148), 3) interviewing skills and supervision (12.8%, N=124) and 4) Basic quality education (12.3 %, N=119) for the human rights and client-focused intervention is 3rd and 4th needs. Conclusions: The findings suggest that the development of training programs will be needed considering their needs such as strengthening the professionality of each service provider in Korea.